Real-time imaging of photodynamic action in bacteria

26 Apr '17 | By Biophotonics.World

Image source: Journal of Biophotonics

Fluorescence imaging studies of the processes leading to photodynamic inactivation of bacteria have been limited due to the small size of microorganisms as well as by the faint fluorescence of most photosensitizers. A versatile method based on highly-sensitive fluorescence microscopy is presented which allows to study, in real time, the incorporation of photosensitizers inside S. aureus upon photodynamic action. The method takes advantage of the fluorescence enhancement of phenothiazine and porphyrin photosensitizers upon entering the bacterial cytosol after the cell wall has been compromised. In combination with typical assays, such as the addition of specific enhancers of reactive oxygen species, it is possible to extract mechanistic information about the pathway of photodynamic damage at the single-cell level. Imaging experiments in deuterated buffer strongly support a Type-I mechanism for methylene blue and a very minor role of singlet oxygen. The article was published by Anita Gollmer et al. in J. Biophotonics, 2017, 2, 264; DOI: 10.1002/jbio.201500259

Methods and Techniques: Endoscopy, General microscopy (white light, confocal, bright field, dark field, phase contrast, DIC etc.), Linear and non-linear fluorescence imaging (confocal LSM, multi-photon, STED, PALM, STORM, SIM, FRET, FRAP, FLIM, etc.), Linear and non-linear vibrational microscopy / imaging (IR, confocal Raman, CARS, SRS etc.), Near-field microscopy (SNOM, AFM, STM, etc.), Optical Coherence Tomography (OCT ), Operating microscopy, Photoacoustic imaging (PAI, MSOT), Polarimetry, Probe and sensor development, Terahertz imaging, Thermography, Microscopy/Imaging, other methods and techniques

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Comments:

19 Dec '22

hairga teway

An original innovation is an actual method. Wonderful. By damaging the bacterial cell wall, phenothiazines and porphyrins have the capacity to penetrate the bacterial cytoplasm and enhance fluorescence in https://drivemad.io/An original innovation is an actual method. Wonderful. By damaging the bacterial cell wall, phenothiazines and porphyrins have the capacity to penetrate the bacterial cytoplasm and enhance fluorescenc...See more

21 Mar '23

Rouzman Dallin

Greffe de Cheveux Istanbul https://www.medhairclinic.com/fr/

15 Apr

finn adams

The method's strength lies in its ability to provide detailed insights into the mechanisms of photodynamic damage at the single-cell level. It also integrates well with conventional assays, including those that involve the use of enhancers to increase the production of reactive oxygen species. Significantly, the study's findings support a Type-I photodynamic reaction mechanism for methylene blue, indicating a predominant role for electron transfer or hydrogen abstraction over the involvement of singlet oxygen, which plays a minimal role in this context. This kind of mechanistic insight is crucial for advancing our understanding of bacterial inactivation via photodynamic processes.

https://geekydane.com/midjourney-ai/The method's strength lies in its ability to provide detailed insights into the mechanisms of photodynamic damage at the single-cell level. It also integrates well with conventional assays, including ...See more

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